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wils0645
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Anyone know the Ka for Phosphate Buffered Saline (pH 7.4). I have heard that it is 6.2E-8, but the formula says .008M Na2HPO4, .002M KH2PO4. Using Henderson Haselbalch, [H+] = Ka[KH2PO4]/[Na2HPO4] using pH of 7.4, Ka = 1.59E-7. Which one is correct?