Claude Bile said:Fluorescence occurs due to real electronic transitions, while Raman scattering occurs as a result of virtual electronic-vibrational transitions.
That is, fluorescence is an absorption/re-emission process while Raman scattering is an inelastic scattering process.
Claude.
fsonnichsen said:Claude's answer brings up an interesting point about this problem: when does a photon "lose its identity" and become classified as a "re-emitted" photon?
The main difference between Raman stokes and fluorescence is the type of energy involved in each process. Raman stokes involves inelastic scattering of light, where the scattered photons have a different energy than the incident photons. On the other hand, fluorescence involves the emission of photons with lower energy than the incident photons.
The spectra of Raman stokes and fluorescence differ in terms of the wavelengths of the emitted light. Raman stokes spectra have peaks at longer wavelengths than the incident light, while fluorescence spectra have peaks at shorter wavelengths than the incident light.
In general, Raman stokes is more sensitive for detecting molecules than fluorescence. This is because Raman stokes involves the detection of scattered photons, which can be amplified by using a sensitive detector. Fluorescence, on the other hand, involves the detection of emitted photons, which can be affected by factors such as photobleaching.
Yes, Raman stokes and fluorescence have different applications in scientific research. Raman stokes is commonly used for analyzing the molecular structure and composition of a sample, while fluorescence is often used for studying the dynamics and interactions of molecules in a sample.
Yes, Raman stokes and fluorescence can occur simultaneously in a sample. This is called Raman-fluorescence or Raman-induced fluorescence and can provide complementary information about the sample. However, the intensity of the fluorescence signal may be stronger than the Raman signal, making it difficult to detect the Raman stokes signal.