X-ray crystallography - Electron-density map

In summary, the individual is seeking assistance with understanding why an electron-density map (EDM) is used in X-ray crystallography experiments for biomolecule structure determination, rather than directly using diffraction data. They have done research and have not found a clear explanation for this method. They are hoping for clarification on the concept and any additional help would be appreciated.
  • #1
TanGeriN
5
0

Homework Statement



Hello, i have the following task in my homework:

When doing an X-ray crystallography experiment to determine the structure of biomolecules (protein/DNA), why do consider interpreting an electron-density map (EDM) instead of directly using the diffraction data?

2. The attempt at a solution

I searched and read through a lot of papers and other sources of information, but in all of them an EDM is created to construct an all atom model of the desired biomolecule. What is meant with "directly using diffraction data" ? As far as i understood, all you have is amplitude and (approximated) phase information after analyzing the series of diffraction patterns. What other way exists for structure determination besides creating an EDM as the Fourier transform of amplitudes and phases?
I hope that someone can help me with this task.

Thank you very much in advance for any kind of help​
 
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  • #2
I'm sorry you are not finding help at the moment. Is there any additional information you can share with us?
 

Related to X-ray crystallography - Electron-density map

1. What is X-ray crystallography?

X-ray crystallography is a technique used to determine the atomic and molecular structure of a crystal. It involves shining X-rays onto a crystal and analyzing the diffraction patterns produced to create a 3D image of the crystal's structure.

2. How does X-ray crystallography work?

X-rays are directed onto a crystal, and as they pass through the crystal, they are diffracted by the atoms within the crystal. The diffracted X-rays are captured on a detector, and the resulting diffraction pattern is used to determine the positions of the atoms in the crystal.

3. What is an electron-density map?

An electron-density map is a 3D representation of the electron density within a crystal. It is created using the diffraction data obtained from X-ray crystallography and shows the positions of the atoms within the crystal.

4. How is an electron-density map used in X-ray crystallography?

The electron-density map is used to determine the positions of the atoms within the crystal, as well as their chemical bonding and orientation. It also provides information about the arrangement of molecules within the crystal and can help determine the overall structure of the crystal.

5. What are the applications of X-ray crystallography?

X-ray crystallography is used in a variety of fields, including chemistry, biology, materials science, and pharmaceuticals. It is used to determine the structures of proteins, DNA, and other biomolecules, as well as to study the properties and interactions of various materials.

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