- #1
TytoAlba95
- 132
- 19
I had to prepare 1mg/ml BSA for Total protein estimation by Lowry method. I was suggested to add 1ml distilled water to 1mg of BSA in a 2 ml ependoff tube, instead of adding distilled water upto the 1ml mark. The explanation that was given to me was, that, 1mg is a very small amount and it doesn't affect the total volume, much if we add that to 1ml of dH2O. Also as BSA crystals doesn't dissolve easily, and forms froth in the process of dissolving, it becomes more difficult to add water till the1ml mark, as the miniscus isn't formed.
So my question, is the rationale correct? (It sounds okay to me)
Should I be doing this for all solutions where the amount of solute is very less (<1g?)?
So my question, is the rationale correct? (It sounds okay to me)
Should I be doing this for all solutions where the amount of solute is very less (<1g?)?
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