- #1
The Alchemist
- 18
- 0
Hi,
I'm doing some calculations on fluorescence and I'm a little stuck on statistics.
Let's say I need to measure 20 seconds to collect 10000 photons from a single molecule.
Then I can say well I want to measure at most 1 second and therefore I need 20 molecules in order to collect 10000 photons in that second.
Is this way of thinking reasonable? Or do I need to take into account some statistics with S/N ratio and say: Poisson distribution.
I can't find any sources to found this reasoning.
Thanks in advance,
I'm doing some calculations on fluorescence and I'm a little stuck on statistics.
Let's say I need to measure 20 seconds to collect 10000 photons from a single molecule.
Then I can say well I want to measure at most 1 second and therefore I need 20 molecules in order to collect 10000 photons in that second.
Is this way of thinking reasonable? Or do I need to take into account some statistics with S/N ratio and say: Poisson distribution.
I can't find any sources to found this reasoning.
Thanks in advance,