How Do I Calculate Column Cross-Sectional Area for Chromatography Scaling Up?

In summary, the conversation is about calculating the column cross-sectional area for chromatography. The participant is looking for help on how to calculate the area when given the radius and height of the column. One suggestion is to use the formula for the area of a circle (pi * r^2).
  • #1
sotellme
75
0
hi guys!

i am doing the chromatography. in scaling up i have to increase my volumetric flow rate but maintain my linear flow rate. my problem is i don't know how to calculate the column cross sectional area (cm2). i hope you guys who work with this area can tell me how i can calculate it when the radius of the column is 3cm and the height is 15cm.


Linear flow rate (cm/hr) = volumetric flow rate (cm3/hr) /
column cross-sectional area (cm2)



thanks a bunch!
 
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  • #2
Cross section of a column should just be area of a circle. pi*r^2
...unless I'm misunderstanding something about your question.
 
  • #3
thanks Moonbear! i think you are right!
 

Related to How Do I Calculate Column Cross-Sectional Area for Chromatography Scaling Up?

1. What are common causes of poor peak shape in chromatography?

The most common causes of poor peak shape in chromatography include issues with the column, such as blockages or voids, incorrect mobile phase composition or flow rate, and sample overload.

2. How can I troubleshoot baseline drift in my chromatography results?

Baseline drift in chromatography can be caused by several factors, including temperature fluctuations, air bubbles in the mobile phase, and issues with the detector. To troubleshoot this problem, check for any temperature changes in the instrument, degas the mobile phase, and clean the detector according to the manufacturer's instructions.

3. What is the ideal sample concentration for chromatography analysis?

The ideal sample concentration for chromatography analysis depends on the type of chromatography being performed and the specific analyte being studied. In general, a concentration of 0.01-10 mg/mL is recommended for liquid chromatography, while gas chromatography may require lower concentrations in the range of 0.01-1 mg/mL.

4. Why am I seeing ghost peaks in my chromatography data?

Ghost peaks in chromatography data can be caused by a number of factors, including impurities in the mobile phase or sample, sample contamination, or issues with the column. To troubleshoot this problem, try changing the mobile phase or using a different column. If the issue persists, it may be necessary to clean or replace the column or check for sample contamination.

5. Can I reuse chromatography columns?

In most cases, chromatography columns cannot be reused due to the risk of cross-contamination and loss of column performance. However, some columns may be able to be regenerated or reconditioned for reuse, depending on the type of column and the analytes being studied. It is best to consult the manufacturer's instructions for specific guidelines on reusing chromatography columns.

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