How can I improve my HPLC method for beta-alanine?

[Rayyana123]

I am working on HPLC method Beta alanine and unable to get a good enough peak. I'm using an agilent system HPLC ,gradient mobile phase(a: 0.1% formic Acid in Ro water,0.1% Formic acid in ACN) 250 mm C18 4/6x 250 mm column Flow rate 1.0 ul Detector: 214 nm 10 ul injected temp:ambient
Gradient below min A: B: 0 95 5 2 75 25 10 50 50 12 5 95 15 5 95 Im unsure on what I'm not doing correctly! Any help will be appreciated!

Reference: https://www.physicsforums.com/forums/chemistry.83/

 

[Adrmay]

Some suggestions to improve your HPLC method:1. Increase the column temperature to 40°C. This will help to improve peak resolution and reduce the retention time of the analyte, increasing peak efficiency.2. Increase the flow rate to 1.2 mL/min or higher. This will help to improve peak resolution and reduce the retention time of the analyte, increasing peak efficiency.3. Reduce the concentration of formic acid in the mobile phase to 0.05%. This will reduce the peak tailing of the analyte, improving peak shape.4. Adjust the gradient program. Try increasing the slope of the gradient or trying different combinations of solvents to find the optimum conditions for the separation of the analyte.5. Check the quality of the column. Make sure that it is not blocked or damaged. Replace the column if necessary.