- #1
Ahmed Abdullah
- 203
- 3
I want to know what is happening at micro-level, how macroscopic tumbling of the solution can actually break a chemical bond?
Can you explain how vigorous vortex-ing can damage DNA?
In summary, the conversation discusses the effects of vigorous vortexing on breaking chemical bonds, specifically in DNA and protein molecules. It is suggested that the different speeds of different parts of the vortex can create forces on the molecules, potentially leading to bond breakage. The compact structure of DNA and proteins, such as in chromosomes, is also considered and whether or not vortexing can break these structures. There is also mention of denaturing and foam formation as potential factors in bond breakage.
- #2
Borek
Mentor
- 28,959
- 4,264
Mechanically - it is not different to what happens when you break the stick.
- #3
Ahmed Abdullah
- 203
- 3
In breaking a stick it is easy to see what are the forces and how they are working. what are the forces involved in breaking of a DNA strand by vortexing. Do the collision rate and energy increase during vigorous vortexing of the solution?
- #4
Borek
Mentor
- 28,959
- 4,264
Different parts of the vortex move at different speeds, DNA molecules are quite long, if different parts of the molecule move at different speeds, it means forces acting on the molecule.
- #5
Ahmed Abdullah
- 203
- 3
Borek said:
That make sense.
- #6
Ahmed Abdullah
- 203
- 3
Borek said:
DNA is a very long molecule but it become very small when packaged into chromosome. Chromosome is not only DNA but also protein packaged together. Do you think vigorous vortexing can break compact structure like chromosome? (Vigorous vortexing in usual sense)
- #7
Ahmed Abdullah
- 203
- 3
I am quite surprised that vortexing can break any bond at all. DNA is an extraordinary long molecule .. that's why it is conceivable . Do protein break in this way? Any example of protein breakage by vortex (not denaturing which can occur because of foam formation during vortex-ing).
Related to Can you explain how vigorous vortex-ing can damage DNA?
1. How does vigorous vortex-ing damage DNA?
When a sample containing DNA is placed in a vortex and spun at high speeds, the force generated can cause shearing and breaking of the DNA strands. This is because the DNA strands are delicate and can easily be damaged by physical forces.
2. Is vigorous vortex-ing the only way DNA can be damaged?
No, there are other ways in which DNA can be damaged, such as exposure to radiation, chemicals, or extreme temperatures. However, vigorous vortex-ing is a common laboratory technique that can cause damage if not done carefully.
3. How can one prevent DNA damage during vortex-ing?
To prevent DNA damage during vortex-ing, it is important to use the correct speed and time parameters for the sample being tested. It is also recommended to use specialized tubes or microcentrifuge tubes designed for vortex-ing, as they can help protect the DNA from excessive shearing forces.
4. What are the consequences of DNA damage caused by vigorous vortex-ing?
The consequences of DNA damage can vary, depending on the extent of the damage. In some cases, it may lead to inaccurate or inconclusive results in experiments, while in others it may cause mutations or completely destroy the DNA, rendering it unusable for further analysis.
5. Is there a limit to how long DNA can be vortex-ed without causing damage?
The duration of vortex-ing can also play a role in DNA damage. While shorter vortex-ing times may not significantly affect DNA, longer periods of vortex-ing can increase the risk of damage. It is important to follow the recommended protocols and time limits for vortex-ing to minimize the risk of DNA damage.
Similar threads
-
Chemistry
-
Chemistry
-
Chemistry