Bio-Gel P2 is a type of gel filtration medium, used for separating and purifying molecules based on their size.
"Cut off 2.000 daltons" refers to the molecular weight limit of the molecules that can be separated using Bio-Gel P2. This means that any molecules with a molecular weight above 2,000 daltons will not be able to pass through the gel and will be retained, while smaller molecules will pass through and be collected in the filtrate.
Bio-Gel P2 is commonly used in protein purification and analysis, as well as in separating and purifying other biomolecules such as nucleic acids and carbohydrates. It is also used for desalting and buffer exchange in biochemical and biotechnological applications.
One of the main advantages of Bio-Gel P2 is its high resolution and efficiency in separating molecules of different sizes. It is also stable and reproducible, making it a reliable tool for research. Additionally, Bio-Gel P2 is compatible with a wide range of buffers and can be easily regenerated for multiple uses.
While Bio-Gel P2 is a versatile tool, it does have some limitations. It is not suitable for separating molecules with very similar molecular weights, as they may overlap and not be effectively separated. Additionally, Bio-Gel P2 is not suitable for purifying very large molecules, as they may become trapped in the gel matrix and not elute properly.