I don't believe that temperature is really necessary, but just preferable because it can make the whole reaction go faster.
Originally it was not used at such temperature because the polymerase would denature at such high temperatures, then polymerases were adapted from thermophilic archaea...
edit: Nevermind, I realized a way to find the answer after posting it. Though I still don't know about the thing involving the notes, can someone confirm if what I have written down as my teacher doing is true?
Homework Statement
note: I'll use this S as an integral symbol.
S(ln...
I already know the Ka for acetic acid, it's .000017. I solved the equation for x, which was almost nothing, and wound up with about the same pH as if I didn't add x to the H3O.
I've never heard of a ICE equation before, could you explain what that is please?
Homework Statement
I'm making a table on excel with the calculated pH of the solution vs the actual measured pH from the lab. My problem is finding the pH of a acetic acid-acetate buffer that has been titrated with HCl, once the moles of HCl are greater than the initial quantity of mols of...
I'd really appreciate help :)
Homework Statement
A 0.05 M aqueous solution of sodium hydrogen sulfate, NaHSO4, has a pH of 1.73. Calculate Ka2 for sulfuric acid. Sulfuric acid is a strong electroylyte, so you can ignore the hydrolysis of the HSO4- ion.
Homework Equations
If I knew that I'd...