Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote.
So did anyone has taken the research on control the protein phosphorylation in wildtype microorganisms with...
But as what I observe in my experiment,there are a lot of bacteria in dividing phase couldn't be stained by SYTO13,and what I wonder is whether the DNA structure or I should say the density of DNA and its binding protein in dormant bacteria greatly effect the affinity between DNA and nucleic dye?
I found that it's difficult to stain dormant bacteria or bacteria in lag phase with Nucleic acid dye like SYTO9/SYBR Green Ⅰ, does anyone know why?
DNA 3D structure too complex? DNA binding protein too much? Low material transport efficiency in bacteria? Or anyother factors?
Is there any...