Thank you for your reply!
This is a very detailed description of lycopene extraction from tomatoes. But it will not be of much use to me.
Extraction of lycopene form bacterium is much easier, and i have read articles about it. I just need to add solvent, incubate and centrifuge pellet.
My main...
I have used Acetone, to extract Lycopene from bacteria, as described in literature. But when I started to measure the absorbance (470 nm) using 96-well microplates, some of the acetone would evaporate and ruin my results (even with the lid on).
Most of the literature that i have read uses HPLC...