Recent content by koitk

  1. koitk

    Need Tips and Tricks on plate-reading Lycopene production of E.coli

    Thank you for your reply! This is a very detailed description of lycopene extraction from tomatoes. But it will not be of much use to me. Extraction of lycopene form bacterium is much easier, and i have read articles about it. I just need to add solvent, incubate and centrifuge pellet. My main...
  2. koitk

    Need Tips and Tricks on plate-reading Lycopene production of E.coli

    I have used Acetone, to extract Lycopene from bacteria, as described in literature. But when I started to measure the absorbance (470 nm) using 96-well microplates, some of the acetone would evaporate and ruin my results (even with the lid on). Most of the literature that i have read uses HPLC...
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