This is my first time isolating the whole genomic DNA from cells. I have two cell line HeLa and Human fibroblasts (almost fully confluent T-25 flasks). The problem is that the protocol which is to be followed for genomic dna extraction is only suitable for DNA solutions less than or equal to...
Can anyone explain mock methylation. The best explanation that I've been able to coax out of the internet is (incubation with methylase in the absence of SAM (S-adenosyl methionine)). I don't know how this sort of methylation gets done, what gets added onto cytosine and how mock methylation...
When performing transformation, after adding SOC media to the newly transformed cells, we place them at 37 degrees Celsius for an hour to allow growth. I understand the need for an incubator but I'm confused regarding how shaking helps in microbial growth? I did some searching on the internet...
Could someone explain what network motifs are and how they are different from clusters because wherever I've searched, both are defined as sub-communities within a network.
The coefficient of variation tells us about the consistency in the data. I know that the lower the coefficient of variation is, the higher will be the consistency in the data. What I don't understand is what is being meant by 'consistency' here. Could someone please explain that?
Which type of GABA receptors are present on dendritic spines? Also where are they present on the dendritic spine (i.e. head, neck or shaft)? Also, how do these GABA receptors contribute to dendritic spine plasticity?
Any references would be greatly appreciated.
Normally when we have a FASTA header on top of a sequence of a gene in ncbi you expect something like
>chromosome:NCBI36:7:100155759:100159857:1
This clearly indicate that on the 7th chromosome and between bases 100155759 and 100159857 the concerned gene is present. It further tells us...
Accessing revision history is simple. After accessing an entry, you go to the top left side of the page and in display settings you select revision history and press ok.
And maybe you're right with regards to revisions being updates on the annotations. It does make sense. But I need to...
I'm not really sure regarding the direction in which the ions flow during re-polarization but you got to understand two things.
In active transport ions are transported against their concentration gradients and because of that energy (in the form of ATP) is used.
Okay so I'm using ncbi and when using the nucleotide database, when I access the revision history of a result of any query, I get a lot of sequences. Surprisingly they all are of the same version. Why? If each is different shouldn't they have different version numbers? They all have different...
Suppose we have a sequence of nucleotides 1 - 1430.
The gene is from 606 - 1430.
The mRNA is from 606-1170. So is the exon (i.e. its 606-1170).
The CDS is from 990-1170.
What would be the location of the 5' and 3' UTR's?
I think the 5' UTR would be 606-990 and the 3' UTR would be...
I don't get what they are. My professor said that they were receptors (present in the cell membrane) for different microorganisms but I just can't understand it because my understanding of a receptor is like a receptor for a hormone. Do sterols work similarly?