If an endoprotease is known to cleave "at" a particular residue - for instance "at" S102 - does it cleave N-terminally or C-terminally of S102? In other words, is the residue which the enzyme cleaves "at" retained in the modified protein or lost along with the 'waste'?
Thanks for any help.
Hi epenguin, thanks for the reply.
I was referring to a protease - the RNA is only there to activate the protease. I'm just wondering whether the mechanism offered by RNA could be replaced by an internal mechanism for activation within the protein that is not dependent on RNA being there.
As...
I've been given an 'assignment' of sorts (more of a thought exercise really) to mutate specific amino acid residues in a viral protein (from poliovirus) such that it can catalyse its own cleavage independently of RNA.
I've learned from my own research that the usual cleavage mechanism...
t(4;11)(p16;p15)
Hi, what would the above mean in terms of chromosome diagnosis? I understand a translocation has occurred between chromosomes 4 and 11, but not what (p16;p15) means.
Thanks.
You've got me wrong there - I'm as anti-creationist as the next sensible person. I'm just trying to get my head around this.
I understand that non-specialised cells have all the latent capabilities of specialised ones - but this is only the case if those capabilities are written into the...
But the individual cells in contact with the environment aren't the ones moving away/towards a stimulus anymore - in a multicellular organism, movement requires that sensory cells can detect a stimulus and a cooperation of cells in the brain/CNS to receive information of the stimulus and mediate...
Thanks for your reply,
I thought of chemotaxis - but then I wondered how this applies to multicellular organisms whose chemotactic ability depends on the correct differentiation of many cells and complex intercellular communication between them.
Given that 'chemotaxis' in humans involves a...
OK...I'm not sure how well I'll word this or if it's a coherent idea biologically but I'll throw it out there anyway...
Regarding the evolution of senses - let's take the detection of sour tastes as an example. I know little neuroscience but I know that there's the involvement of H+ ions as the...
Hello,
I have an upcoming exam on human genetics and genetic techniques and I am trying to learn about this procedure. I have searched the internet but come up with more studies that have used this technique rather than a plain 'for dummies' explanation of what it is. If someone could offer...
Hi guys,
Got a semester exam on Population Genetics coming up in a few weeks and I'm really struggling to get my head around this concept.
I've read a number of internet sources but am still not completely certain of what it is. Could anyone give me a basic overview, also I've got it...
Does mtDNA recombine? I've tried doing my own research but I'm getting very mixed responses, from the evidence being strongly against it, right up to it being a well-known phenomenon in yeast. any insight please?
I'm a little confused about what the ΔΔCt value means and how its calculated - could anyone verify?
Also, I'm reading lecture notes that say "2^ΔΔCt assumes efficiency is 100%". I'm really baffled by this and would appreciate any guidance.
Yeah but that's what I said - there is no direct requirement for carbohydrates themselves, only for the energy which carbohydrates (among other nutrients) can provide. Incidentally, though, the molecule fed into the Krebs cycle is acetyl coA which is a product of fatty acid oxidation as well as...